UvA - DARE ( Digital Academic Repository ) Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae

Extension of a reporter protein with the carboxyterminal thirty amino acids of the cell wall mannoprotein a-agglutinin of Saccharomyces cerevisiue resulted in incorporation of the chimeric protein in the cell wall. By Western analysis it was shown that the incorporated protein contained /I-1,6-glucan similar to endogenous cell wall proteins, whereas excreted reporter protein was not glucosylated. This suggests that B-1,6-glucan is involved in anchoring mannoproteins in the cell wall.